Big Sister may refer to:
An older sister, see Birth order
Big Sister (software), network monitoring software
Big Sister (brothel), an online brothel in Prague
"The Big Sister", an episode of Dexter's Laboratory
"Big Sister," a song by Elvis Costello and the Attractions from Trust (Elvis Costello album)
Big Sister's Island, one of the Sisters' Islands of Singapore
Big Sisters part of the Big Brothers, Big Sisters program.
Big Sister (radio) was a radio soap opera
Big Sister, an enemy type in the game BioShock 2.
Big Sis, a nickname for Secretary of Homeland Security Janet Napolitano
Stóra systir, an anti-prostitution vigilante group in Iceland
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